Blood Brain Interfaces Exploratory Plateform

The BIP core facility develops technological tools to:  

  • study functions of the blood-brain barriers and evaluate neuroprotective strategies restoring barrier efficacy
  • evaluate and decipher the mechanisms controlling the cerebral bioavailability of drugs
  • investigate immune cell trafficking and pathogen invasion into the brain 
Méthodes et techniques

The BIP core facility provides counsulting and various services:

  • in vivo blood-brain and blood-CSF permeability measurements in developing rodent,
  • ex vivo blood-brain interfaces (cerebral microvessels and choroid plexuses) isolation for molecular or functional studies
  • use of differentiated cellular models of blood-brain interfaces for molecular transport and cellular migration studies.  

Understanding blood-brain interface functions is a key issue to improve CNS drug bioavailability, decrease the side-effects of other non CNS drugs and validate therapeutic targets in these interfaces.

Funded by IHU-CESAME, PIA, “Investment for the Future Program”, the core facility BIP develops relevant tools and provides services to explore the functions (and dysfunctions) of blood-brain interfaces.

BIP also builds up a collection of blood-brain interface tissues from rodents and non-human primates for molecular and biochemical analyses, or immunochemistry.

BIP is open to internal teams (CRNL) and external collaborations (academic research labs, biotech and pharmaceutical companies). Services range from fee-for-service to collaborative research contracts. The facility is equipped and has analytical expertise for spectrophotometry, spectrofluorimetry, fluorescence micro- and macroscopy, protein extraction, UV- Fluo-HPLC, radiodetection, RNA isolation and qRT-PCR, Elisa.

Further information can be obtained by downloading this document.