During this PhD, I study the alterations induced by auto-antibodies from Neuromyelitis Optica (NMO) patients, a disease related to multiple sclerosis, on a specific type of glial cells : ependymal cells. These multiciliated epithelial cells lines the ventricles and the spinal cord central canal filled with cerebrospinal fluid (CSF). These cells have vital functions such as the regulation of metabolic exchanges between the CSF and the central nervous system (CNS), the regulation of CSF propulsion and the development of the CNS during early life. Studying the effects of auto-antobodies on these functions will help to better understand the physiopathology of NMO.
Methods used :
- Primary culture and explant cultures of ependymocytes
- In-vivo model of NMO : "NMO-rat"
- Immunohistochemisty and immunocytochemistry
- Videomacroscopy and microscopy
- RNAseq transcriptomic analyses